Prevalence of Intestinal Parasites and the Therapeutic Potential of Andrographis Paniculata Leaf Extract in Managing Coccidiosis and Enhancing Growth Performance in Broiler Chickens
Keywords:
Coccidiosis, Intestinal parasites, Andrographis paniculata, Eimeria tenella, Broiler chickensAbstract
Intestinal parasitic infections remain a persistent challenge in poultry production, with coccidiosis standing out as one of the most economically damaging diseases due to its effects on growth, feed utilization, and flock health. The growing concerns surrounding drug resistance, residue accumulation, and rising costs of conventional anticoccidial agents have intensified the search for safer and more sustainable alternatives. This study investigated the prevalence of intestinal parasites in broiler chickens sourced from selected markets in Uyo metropolis and evaluated the therapeutic efficacy and growth-promoting potential of Andrographis paniculata leaf extract in broilers experimentally infected with coccidiosis. A total of 420 broilers were sampled from Itam and Akpan Andem markets, and fecal samples were analyzed using standard parasitological techniques. Additionally, an experimental trial involving Eimeria tenella-infected broilers treated with graded doses (100, 200, and 400 mg/kg body weight) of the plant extract was conducted, alongside a conventional drug control (diclazuril). Results revealed an overall parasite prevalence of 43.1%, with Eimeria spp. being the most dominant. The extract significantly reduced oocyst shedding and improved growth indices in a dose-dependent manner. These findings highlight the potential of A. paniculata as a viable natural alternative in poultry disease management.
References
Abbas, R. Z., Iqbal, Z., Khan, M. N. & Zafar, M. A. (2011). Anticoccidial activity of herbal complex in broiler chickens challenged with Eimeria tenella. Parasitology Research, 109(3), 727–733.
Abraham, A., Mtewa, A. G., Chiutula, C., Mvula, R. L. S., Maluwa, A., Eregno, F. E. & Njalam’mano, J. (2025). Prevalence of Antibiotic Resistance Bacteria in Manure, Soil, and Vegetables in Urban Blantyre, Malawi, from a Farm-to-Fork Perspective. International Journal of Environmental Research and Public Health, 22(8), 1273.
Akinmoladun, F. O., Akinrinlola, B. L. & Farombi, E. O. (2007). Phytochemical constituents and antioxidant activity of extract from the leaves of Vernonia amygdalina. African Journal of Biotechnology, 6(10), 1196 – 1200.
Andersen, L., Corazon, S. S. & Stigsdotter, U. K. (2021). Nature Exposure and Its Effects on Immune System Functioning: A Systematic Review. International Journal of Environmental Research and Public Health, 18(4), 1416.
Asiru, M. F. & Abifarin, M. O. (2016) Health Promotion through Herb Information: A special discussion of gegemu herb in Yoruba setting of Nigeria. J Health Inform Lib., 3 (1): 41-45.
Attia, Y. A., Rahman, M. T., Hossain, M. J., Basiouni, S., Khafaga, A. F., Shehata, A. A. & Hafez, H. M. (2022). Poultry Production and Sustainability in Developing Countries under the COVID-19 Crisis: Lessons Learned. Animals, 12(5), 644.
Badparva, E. & Kheirandish, F. (2017). Epidemiology of pathogenic parasite Histomonas meleagridis in poultry in Lorestan province, western Iran. J Parasit Dis. 2017 Dec;41(4):1040-1043.
Baeshen, N. A., Almulaiky, Y. Q., Afifi, M., Al-Farga, A., Ali, H. A., Baeshen, N. N., Abomughaid, M. M., Abdelazim, A. M., & Baeshen, M. N. (2023). GC-MS Analysis of Bioactive Compounds Extracted from Plant Rhazya stricta Using Various Solvents. Plants, 12(4), 960.
Caisin, L. & Scripnic, E. (2026). Poultry Farming in the Republic of Moldova: Current Trends, Best Practices, Product Quality Assurance, and Sustainable Development Strategies. Sustainability, 18(2), 626.
Cantin-Rosas, B., Tomazic, M. L., Rodríguez, A. E., Enciso, N., Brante-Bernier, J., Honores, P., Godoy-Alfaro, C., Abarca, C., Alegría-Morán, R. & Ramirez-Toloza, G. (2025). Risk Factors and Spatial Distribution of Gastrointestinal Parasites in Backyard Poultry Production Systems in Central Chile. Veterinary Sciences, 12(5), 448.
Chaachouay, N., & Zidane, L. (2024). Plant-Derived Natural Products: A Source for Drug Discovery and Development. Drugs and Drug Candidates, 3(1), 184-207.
Chapman, H. D. (2009). A landmark contribution to poultry science prophylactic control of coccidiosis in poultry. Poultry Science, 88(4), 813–815.
Choi, J., Ko, H., Tompkins, Y. H., Teng, P.-Y., Lourenco, J. M., Callaway, T. R. & Kim, W. K. (2021). Effects of Eimeria tenella Infection on Key Parameters for Feed Efficiency in Broiler Chickens. Animals, 11(12), 3428.
Efriem, S., Sabastian, C., Blum, S., Fleker, M., Mabjeesh, S. J., & Britzi, M. (2023). Resistant Bacteria in Broiler Litter Used as Ruminant Feed: Effect of Biotic Treatment. Antibiotics, 12(7), 1093.
George, N. J., Ibanga, J. I. & Ubom, I. A. (2015). Geoelectrohydrogeological indices of evidence of ingress of saline water into freshwater in parts of coastal aquifers of Ikot Abasi, southern Nigeria, Journal of African Earth Sciences, 109, 37–46.
George, N. J., Ubom, A. I. & Ibanga J. I. (2014). Integrated Approach to Investigate the Effect of Leachate on Groundwater around the Ikot Ekpene Dumpsite in Akwa Ibom State, Southeastern Nigeria. International Journal of Geophysics, 2014, Article ID 174589, 1 - 12.
Islam, M. S., Mir, D. A., Islam, S., Jahid, M. A., Sumona, N. A., Swar, D. K., Akter, N., Zaman, S., Khatun, M. M., Islam, M. A., Lovelu, M. A. & Nazir ME (2025). Prevalence of Coccidiosis in Commercial Chickens of Bangladesh: Species Identification with Age and Seasonal Associations. World Vet. J., 15(3): 764-773.
Kabeta, T., Tolosa, T., Nagara, A., Chantziaras, I., Croubels, S., Van Immerseel, F. & Antonissen, G. (2024). Awareness of Poultry Farmers of Interconnected Health Risks: A Cross-Sectional Study on Mycotoxins, Biosecurity, and Salmonellosis in Jimma, Ethiopia. Animals, 14(23), 3441.
Kovács, L., Domaföldi, G., Bertram, P.-C., Farkas, M., & Könyves, L. P. (2025). Biosecurity Implications, Transmission Routes and Modes of Economically Important Diseases in Domestic Fowl and Turkey. Veterinary Sciences, 12(4), 391.
Lee, Y., Lu, M. & Lillehoj, H. S. (2022). Coccidiosis: Recent Progress in Host Immunity and Alternatives to Antibiotic Strategies. Vaccines, 10(2), 215.
Liu, S., Sun, L., Zhang, P., & Niu, C. (2024). Recent Advances in Grayanane Diterpenes: Isolation, Structural Diversity, and Bioactivities from Ericaceae Family (2018–2024). Molecules, 29(7), 1649.
López-Abán, J., Vicente-Santiago, B., Gutiérrez-Soto, G., Rodríguez-Garza, N. E., Kačániová, M., López-Sandin, I., Romo-Sáenz, C. I., Ballesteros-Torres, J. M., Galaviz-Silva, L., Castillo-Velázquez, U., Garzoli, S. & Elizondo-Luévano, J. H. (2025). Emerging Approaches to Anthelmintic Therapy
Using Medicinal Plants and Phytochemicals: A Review of Natural Products Against Strongyloidiasis. Pathogens, 14(9), 842.
Mahizan, N. A., Yang, S.-K., Moo, C.-L., Song, A. A.-L., Chong, C.-M., Chong, C.-W., Abushelaibi, A., Lim, S.-H. E., & Lai, K.-S. (2019). Terpene Derivatives as a Potential Agent against Antimicrobial Resistance (AMR) Pathogens. Molecules, 24(14), 2631.
Martins, A. J. d. A., Rocha, A. C. d. S., Assis-Silva, Z. M. d., Maia, G. O., Alves-Ribeiro, B. S., Duarte, R. B., Moraes, I. d. S., Urzedo, N. F. d., Fernandes-Silva, L., Gomes, A. P. C., Felizarda, S. M., Oliveira, M. P., Saturnino, K. C., Rezende, H. H. A., Rodrigues, R. M., Ramos, D. G. d. S. &
Braga, Í. A. (2025). Risk of Environmental Contamination by Gastrointestinal Parasites in Public Areas of the Central Plateau Microregion of Brazil: A Public Health Concern. Pathogens, 14(3), 211.
Martins, R. R., Silva, L. J. G., Pereira, A. M. P. T., Esteves, A., Duarte, S. C. & Pena, A. (2022). Coccidiostats and Poultry: A Comprehensive Review and Current Legislation. Foods, 11(18), 2738.
Marzouk, E. & Alajaji, A. I. (2025). Preventive Immunology for Livestock and Zoonotic Infectious Diseases in the One Health Era: From Mechanistic Insights to Innovative Interventions. Veterinary Sciences, 12(10), 1014.
McDougald, L. R. & Fitz-Coy, S. H. (2013). Coccidiosis. In D. E. Swayne (Ed.), Diseases of Poultry (13th ed., pp. 1148–1165). Wiley-Blackwell.
Murshed, M., Aljawdah, H. M. A., Mares, M. & Al-Quraishy, S. (2023). In Vitro: The Effects of the Anticoccidial Activities of Calotropis procera Leaf Extracts on Eimeria stiedae Oocysts Isolated from Rabbits. Molecules, 28(8), 3352.
Naidoo, V., McGaw, L. J., Bisschop, S. P. R., Duncan, N. & Eloff, J. N. (2008). The value of plant extracts with antioxidant activity in attenuating coccidiosis in broiler chickens. Veterinary Parasitology, 153(3-4), 214–219.
Oliveira, S. M. R., Girol, A. P., Nissapatorn, V. & Pereira, M. d. L. (2025). Bioactive Compounds Derived from Plants and Their Medicinal Potential. Pharmaceuticals, 18(11), 1732
Ratcliffe, N. A., Mello, C. B., Castro, H. C., Dyson, P., & Figueiredo, M. (2024). Immune Reactions of Vector Insects to Parasites and Pathogens. Microorganisms, 12(3), 568.
Riber, A. B. & Wurtz, K. E. (2024). Impact of Growth Rate on the Welfare of Broilers. Animals, 14(22), 3330.
Rizwan, H. M., Zohaib, H. M., Sajid, M. S., Abbas, H., Younus, M., Farid, M. U., Iftakhar, T., Muzaffar, H. A., Hassan, S. S., Kamran, M., Raza, M. H. &
Bajwa, M. H. (2023). Inflicting Significant Losses in Slaughtered Animals: Exposing the Hidden Effects of Parasitic Infections. Pathogens, 12(11), 1291.
Rosłoń, M., Majewski, E., Gębska, M., Grontkowska, A., Motrenko, M., Żbikowski, A., Michalczuk, M. & Urban, J. (2025). Vaccinations and Functional Feed Supplements as Alternatives to Coccidiostats for the Control of Coccidiosis in Raising Broiler Chickens. Animals, 15(17), 2548.
Thrusfield, M., Christley, R., & Brown, H. (2018). Veterinary Epidemiology (4th ed.). Wiley-Blackwell.
Xu, H., Lan, S., Lin, S., Wang, A., Luo, Y., Wang, J. & Yang, Z. (2025). Exploring the Active Constituents of Andrographis paniculata in Protecting the Skin Barrier and the Synergistic Effects with Collagen XVII. Antioxidants, 14(1), 118.
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